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KMID : 1144320120440010005
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감염과 화학요법
2012 Volume.44 No. 1 p.5 ~ p.10
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Molecular Epidemiologic Analysis of Community-Onset Extended Spectrum Beta-Lactamase (ESBL) Producing Escherichia coli Using Infrequent-Restriction-Site Polymerase Chain Reaction (IRS-PCR) with Comparison by Pulsed-Field Gel Electrophoresis (PFGE)
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Byun Ji-Hyun
Yoo Jin-Hong
Park Chul-Min
Lee Dong-Gun
Park Sun-Hee
Choi Su-Mi
Choi Jung-Hyun
Kim Si-Hyun
Kwon Jae-Cheol
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Abstract
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Background: We evaluated the ability of infrequent restriction site-polymerase chain reaction (IRS-PCR) to perform molecular epidemiologic analysis of Community-Onset Extended Spectrum Beta-Lactamase (ESBL) producing Escherichia coli , and also assessed the use of PFGE as an alternative method.
Materials and Methods: IRS-PCR assay was performed using combinations of adaptors for XbaI and HhaI restriction sites on clinical isolates of E. coli (n=51). We compared the discriminatory power, quality and efficiency of IRS-PCR to PFGE.
Results: In E. coli , PFGE discriminated 39 (76.4%) and IRS-PCR discerned 41 (80.3%) of the total 51 strains. It took much less time to complete IRS-PCR (one day) than PFGE (at least 4 days).
Conclusions: IRS-PCR is a more sensitive and rapid alternative to PFGE for molecular epidemiologic analysis of E. coli.
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KEYWORD
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ESBL-producing E. coli, Pulsed-field gel electrophoresis (PFGE), Infrequent restriction site-polymerase chain reaction (IRS-PCR), Genotypin
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